Review



tfap2c knockout cell lines  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc tfap2c knockout cell lines
    Fig. 4. Genetic depletion of <t>TFAP2C</t> in the initial phase of TSLC derivation (a) Schematic overview of molecular analyses of the first step of TSLC derivation. (b) Western blot analysis of TFAP2C in TFAP2C- knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5), with or without AP for 4 days. ACTIN was used as a loading control. (c) Flow cytometric analysis of GATA3 expression in TFAP2C-knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5). Wild-type KhES-1 cells were used as negative controls. (d) Immunofluorescence images of CTB-related markers in TFAP2C knockout clones (T2CKO#3) and parental ESCs (G3KI#2–5). Scale bars, 100 μm.
    Tfap2c Knockout Cell Lines, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tfap2c+knockout+cell+lines/pm40447714-200-2-42?v=Addgene+inc
    Average 93 stars, based on 2 article reviews
    tfap2c knockout cell lines - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Stepwise strategy for generating human trophoblast stem-like cells from embryonic stem cells reveals specific role of TFAP2C in acquiring self-renewability."

    Article Title: Stepwise strategy for generating human trophoblast stem-like cells from embryonic stem cells reveals specific role of TFAP2C in acquiring self-renewability.

    Journal: Scientific reports

    doi: 10.1038/s41598-025-03830-x

    Fig. 4. Genetic depletion of TFAP2C in the initial phase of TSLC derivation (a) Schematic overview of molecular analyses of the first step of TSLC derivation. (b) Western blot analysis of TFAP2C in TFAP2C- knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5), with or without AP for 4 days. ACTIN was used as a loading control. (c) Flow cytometric analysis of GATA3 expression in TFAP2C-knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5). Wild-type KhES-1 cells were used as negative controls. (d) Immunofluorescence images of CTB-related markers in TFAP2C knockout clones (T2CKO#3) and parental ESCs (G3KI#2–5). Scale bars, 100 μm.
    Figure Legend Snippet: Fig. 4. Genetic depletion of TFAP2C in the initial phase of TSLC derivation (a) Schematic overview of molecular analyses of the first step of TSLC derivation. (b) Western blot analysis of TFAP2C in TFAP2C- knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5), with or without AP for 4 days. ACTIN was used as a loading control. (c) Flow cytometric analysis of GATA3 expression in TFAP2C-knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5). Wild-type KhES-1 cells were used as negative controls. (d) Immunofluorescence images of CTB-related markers in TFAP2C knockout clones (T2CKO#3) and parental ESCs (G3KI#2–5). Scale bars, 100 μm.

    Techniques Used: Western Blot, Knock-Out, Clone Assay, Control, Expressing, Immunofluorescence

    Fig. 6. Schematic summary of TSLC derivation from primed human ESCs. TSLC derivation from primed ESCs involves two distinct molecular programs: a BMP-dependent lineage conversion into the trophoblasts, followed by a TFAP2C-dependent acquisition of self-renewal capacity.
    Figure Legend Snippet: Fig. 6. Schematic summary of TSLC derivation from primed human ESCs. TSLC derivation from primed ESCs involves two distinct molecular programs: a BMP-dependent lineage conversion into the trophoblasts, followed by a TFAP2C-dependent acquisition of self-renewal capacity.

    Techniques Used:



    Similar Products

    tfap2c knockout cell lines  (Addgene inc)
    93
    Addgene inc tfap2c knockout cell lines
    Fig. 4. Genetic depletion of <t>TFAP2C</t> in the initial phase of TSLC derivation (a) Schematic overview of molecular analyses of the first step of TSLC derivation. (b) Western blot analysis of TFAP2C in TFAP2C- knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5), with or without AP for 4 days. ACTIN was used as a loading control. (c) Flow cytometric analysis of GATA3 expression in TFAP2C-knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5). Wild-type KhES-1 cells were used as negative controls. (d) Immunofluorescence images of CTB-related markers in TFAP2C knockout clones (T2CKO#3) and parental ESCs (G3KI#2–5). Scale bars, 100 μm.
    Tfap2c Knockout Cell Lines, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tfap2c+knockout+cell+lines/pm40447714-200-2-42?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    tfap2c knockout cell lines - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 4. Genetic depletion of TFAP2C in the initial phase of TSLC derivation (a) Schematic overview of molecular analyses of the first step of TSLC derivation. (b) Western blot analysis of TFAP2C in TFAP2C- knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5), with or without AP for 4 days. ACTIN was used as a loading control. (c) Flow cytometric analysis of GATA3 expression in TFAP2C-knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5). Wild-type KhES-1 cells were used as negative controls. (d) Immunofluorescence images of CTB-related markers in TFAP2C knockout clones (T2CKO#3) and parental ESCs (G3KI#2–5). Scale bars, 100 μm.

    Journal: Scientific reports

    Article Title: Stepwise strategy for generating human trophoblast stem-like cells from embryonic stem cells reveals specific role of TFAP2C in acquiring self-renewability.

    doi: 10.1038/s41598-025-03830-x

    Figure Lengend Snippet: Fig. 4. Genetic depletion of TFAP2C in the initial phase of TSLC derivation (a) Schematic overview of molecular analyses of the first step of TSLC derivation. (b) Western blot analysis of TFAP2C in TFAP2C- knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5), with or without AP for 4 days. ACTIN was used as a loading control. (c) Flow cytometric analysis of GATA3 expression in TFAP2C-knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5). Wild-type KhES-1 cells were used as negative controls. (d) Immunofluorescence images of CTB-related markers in TFAP2C knockout clones (T2CKO#3) and parental ESCs (G3KI#2–5). Scale bars, 100 μm.

    Article Snippet: Generation of TFAP2C knockout cell lines A set of guide RNAs(gRNAs) targeting TFAP2C was designed as illustrated in Supplementary Fig. S5, and the target sequences are shown in Supplementary Table S1. gRNA expression vectors were constructed using the Cas9 sgRNA backbone vector (Addgene, #68463).

    Techniques: Western Blot, Knock-Out, Clone Assay, Control, Expressing, Immunofluorescence

    Fig. 6. Schematic summary of TSLC derivation from primed human ESCs. TSLC derivation from primed ESCs involves two distinct molecular programs: a BMP-dependent lineage conversion into the trophoblasts, followed by a TFAP2C-dependent acquisition of self-renewal capacity.

    Journal: Scientific reports

    Article Title: Stepwise strategy for generating human trophoblast stem-like cells from embryonic stem cells reveals specific role of TFAP2C in acquiring self-renewability.

    doi: 10.1038/s41598-025-03830-x

    Figure Lengend Snippet: Fig. 6. Schematic summary of TSLC derivation from primed human ESCs. TSLC derivation from primed ESCs involves two distinct molecular programs: a BMP-dependent lineage conversion into the trophoblasts, followed by a TFAP2C-dependent acquisition of self-renewal capacity.

    Article Snippet: Generation of TFAP2C knockout cell lines A set of guide RNAs(gRNAs) targeting TFAP2C was designed as illustrated in Supplementary Fig. S5, and the target sequences are shown in Supplementary Table S1. gRNA expression vectors were constructed using the Cas9 sgRNA backbone vector (Addgene, #68463).

    Techniques: